Assembly, function and molecular architecture of the eukaryotic replicative helicase

Perhaps the most fundamental property of all living things is the ability to replicate their DNA, which allows cells to pass on their genetic material from parent to progeny. Accurate DNA replication is essential for genomic stability and organismal wellbeing. Errors in DNA replication are strongly associated with ageing and cancer. The ring-shaped replicative helicase complex MCM2-7 plays a vital role in DNA replication. Dr Speck is investigating the molecular basis of MCM2-7 ring opening using structural, genetic and biochemical techniques to understand how this fundamental molecular machine functions during DNA replication. Understanding the underlying mechanism of how the replicative helicase gains access to DNA, is regulated on DNA and is released from DNA will also aid the development of novel DNA replication inhibitors with therapeutic value.