Spectral Flow Cytometry (SFC) for profiling single cells of the immune system in health and disease
Year of award: 2020
Grantholders
Dr Andrew Filby
Newcastle University, United Kingdom
Dr Caroline Wilson
Newcastle University, United Kingdom
Prof Matthew Collin
Newcastle University, United Kingdom
Prof Matthias Trost
Newcastle University, United Kingdom
Dr Daniel Rico
Newcastle University, United Kingdom
Dr Arthur Pratt
Newcastle University, United Kingdom
Dr Gary Reynolds
Newcastle University, United Kingdom
Prof Muzlifah Haniffa
Newcastle University, United Kingdom
Dr Christopher Duncan
Newcastle University, United Kingdom
Prof Sophie Hambleton
Newcastle University, United Kingdom
Project summary
According to my 9 year old daughter, cytometrists are "cell detectives" who use special machines to interrogate individual cells to find our suspects in a particular disease with a view to stopping or eliminating the criminals. Just like detectives we fingerprint our suspects, but instead of using ink we utilise fluorescent dyes to mark our cells and then pass them one at a time through laser beams to profile and identify the perpetrators. We then check the fluorescent finger print against a database of cell types so we can find out if they have previously been suspected of causing a disease. The spectral flow cytometry technology we are asking for in this application will allow us to be even better cell detectives because we can ask a much larger number of questions of our suspect cells; with much more detail and speed compared to other approaches to catch the villain!