Spectral Flow Cytometry (SFC) for profiling single cells of the immune system in health and disease

Year of award: 2020

Grantholders

  • Dr Andrew Filby

    Newcastle University, United Kingdom

  • Dr Caroline Wilson

    Newcastle University, United Kingdom

  • Prof Matthew Collin

    Newcastle University, United Kingdom

  • Prof Matthias Trost

    Newcastle University, United Kingdom

  • Dr Daniel Rico

    Newcastle University, United Kingdom

  • Dr Arthur Pratt

    Newcastle University, United Kingdom

  • Dr Gary Reynolds

    Newcastle University, United Kingdom

  • Prof Muzlifah Haniffa

    Newcastle University, United Kingdom

  • Dr Christopher Duncan

    Newcastle University, United Kingdom

  • Prof Sophie Hambleton

    Newcastle University, United Kingdom

Project summary

According to my 9 year old daughter, cytometrists are "cell detectives" who use special machines to interrogate individual cells to find our suspects in a particular disease with a view to stopping or eliminating the criminals. Just like detectives we fingerprint our suspects, but instead of using ink we utilise fluorescent dyes to mark our cells and then pass them one at a time through laser beams to profile and identify the perpetrators. We then check the fluorescent finger print against a database of cell types so we can find out if they have previously been suspected of causing a disease. The spectral flow cytometry technology we are asking for in this application will allow us to be even better cell detectives because we can ask a much larger number of questions of our suspect cells; with much more detail and speed compared to other approaches to catch the villain!