Dissecting the molecular basis of eRNA function
Dr Daniel Bose
University of Sheffield
Enhancers are regulatory DNA sequences that control when and in what tissue genes are turned on and off. Only a small amount of the genome contains genes, but much of the genome is transcribed into RNA; thus many RNAs do not code for proteins. I will focus on enhancer RNAs (eRNAs) which are important for turning genes on and off, but it is unclear how they do this.
I will investigate the way eRNAs work. I will look at how they fold up to form different shapes and how this changes the activity of the proteins that turn genes on and off. I will do this in vitro and in cells, looking across the genome. I will also look at the atomic details of how folded eRNAs interact with regulatory proteins.
My research will uncover mechanisms that describe how eRNAs work.