An AI-driven Automated Platform for High-Throughput 5D Fluorescence Imaging and Data Analysis Applied to Cell Signalling and Drug Discovery

Fluorescence microscopy has emerged as one of the most powerful approaches to investigate the fundamental mechanisms of life, including the complex dynamic events allowing our cells to respond to external and internal stimuli like hormones and neurotransmitters, by using fluorescent labels to tag molecules of interest and follow them over time.  

The application of machine learning (ML) and artificial intelligence (AI) to advanced fluorescence microscopy presents immense potential to model and understand the complex mechanisms of cellular signalling with unparalleled depth, paving the way to transformative scientific discoveries and innovative therapies for conditions like heart failure, cancer or diabetes. However, the widespread application of ML/AI to discovery science is severely hampered by a lack of training data resulting from the low-throughput and heavy user-dependence of current advanced microscopy approaches.  

To overcome these limitations, we will develop an innovative, AI-driven, fully automated high-throughput platform, seamlessly integrating high-throughput 5D fluorescence imaging with comprehensive automation, deep learning-based data analysis, and generative modelling of complex cellular structures and biological processes. The platform will be capable of autonomously acquiring and analysing very large datasets of living cells in 5D (3D space, time, and multiple fluorescent labels) at high spatiotemporal resolution.  

Leveraging this platform, we will systematically investigate how G protein-coupled receptors (GPCRs), the largest family of cell receptors deeply involved in human physiology and disease, work with unprecedented detail, addressing fundamental and still unanswered scientific questions and unlocking novel avenues for drug development.